Isolation and Identification of Citrobacter Species and their Antibiotic Susceptibility with Special Reference to Extended Spectrum Beta-Lactamase
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 3, Pages 11282-11288
AbstractBackground:Citrobacter species are frequent nosocomial pathogens , a local or systemic breach in the host defences can allow them to cause a range of infections which include urinary tract infections , neonatal sepsis , pulmonary infections , meningitis and blood stream infections.Citrobacter species are emerging as important nosocomial pathogens . Citrobacter infections are associated with high mortality rate.
Objectives: To determine their antibiotic susceptibility pattern. To detect extended spectrum beta lactamase producing species.
Material and Methods: Various clinical samples received in the central lab of IGIMS Hospital , Patna Bihar. Identification will be done by using standard microbiological techniques. Antibiotic susceptibility test will be performed by Kirby-Bauer Disc diffusion method. Test strains will be pre-incubated in peptone water at 37oC at an optical density of 0.5McFarland standard. This suspension will be used to inoculate strains onto Muller Hinton agar plate by swabbing them with a sterile cotton swab and performing culture as recommended by clinical and laboratory standards institute . The antibiotic discs used for sensitivity testing will be obtained from Hi-Media , India , which will be used to study the sensitivity pattern.
Conclusion: The magnitude of Citrobacter infections has increased over time considering its potential to cause nosocomial infections and the growing numbers of immunocompromised patients in hospitals. C.koseri and C.freundii being the commonest species isolated. They are usually isolated from patients with wound infections, urinary tract infections , respiratory infections and bacteremia. The emergence of drug resistance among the Citrobacter is noteworthy.
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