Assessment of RT PCR diagnostic assays for SARS CoV - 2 detection in a Molecular diagnostic & Research Lab
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 4, Pages 236-253
AbstractBackground: SARS CoV-2 infection took the whole world by storm in the final month of
2019.Different measures have been taken to reduce its spread by timely and accurate detection of
COVID 19(coronavirus disease 2019) infection in suspected patients and theircontacts.
Aim: This study was conducted to assess commercially available five Rt pcr (reverse
transcriptase polymerase chain reaction) kits from different manufacturers available in our center
for diagnostic testing ofSARS CoV-2 infection .94 oropharyngeal clinical samples,
previously confirmed as 64 positive and 30 negative for SARS CoV-2 were extracted and
amplified separately by each of the five Rt pcr kitsand the results compared.
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