SERUM TOTAL SIALIC ACID AND HS –CRP (HIGH SENSITIVE C-REACTIVE PROTEIN) AS MARKERS OF DIABETIC NEPHROPATHY
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 3, Pages 11557-11573
Abstract
Background:Diabetes mellitus has become a global pandemic and is estimated that it will be the leading cause of death as a non communicable disease by 2030.Diabetic nephropathy is a major long-term complication of diabetes mellitus (DM). Type 2 DM is frequently associated with an underlying low grade inflammatory mechanism , but less information is available on the relationship explaining the low-grade inflammation process and development of diabetic nephropathy (DN). The aim of this study is to determine the serum level of high sensitivity C-reactive protein (hsCRP) ,an acute phase reactant and Serum Total Sialic acid levels, a marker of excessive glycation in unregulated glycemic control in Diabetes mellitus patients leading to the complication of diabetic nephropathy .And to compare with the normal subjects and also to study the association between serum hsCRP levels and Serum Total Sialic acid levels.Materials and Methods: 50 patients with Type 2 DM with nephropathy (DN) and 50 patients of Type 2 DM without nephropathy (DM) along with 50 unrelated age and sex- matched healthy controls were included in the study. Plasma fasting and postprandial glucose levels, renal profile (serum creatinine, BUN), and lipid profile, HbA1c and Urinary Microalbumin levels were analysed. Serum TSA test levels and hs-CRP level were evaluated using thiobarbituric acid assay and immunoturbidimetric kit methods respectively.
Results: We observed a higher concentration of Serum Total Sialic acid levels (83.2 ± 6.8 mg/dl) and hs-CRP (3.22 ± 1.48 mg/L) in diabetic nephropathy than the diabetes mellitus group (74.1 ± 6.2 mg/dl and 2.2 ± 1.40 mg/L, respectively). Both Serum Total Sialic acid levels and hs-CRP levels were found significantly correlated with plasma fasting and postprandial blood sugar, hemoglobin A1c, and urine microalbumin levels in both DM and DN groups. Multiple logistic regression analysis showed that both TSA and hs-CRP was independently associated with diabetic nephropathy.
Conclusion: High serum TSA and hs-CRP levels suggests the underlying inflammatory mechanism in the development of microangiopathic complications of T2DM like diabetic nephropathy.
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