European Journal of Molecular & Clinical Medicine

Abstract

INTRODUCTION :
Bacterial infections that cause production of pus are called pyogenic infections. Pyogenic
infections are associated with high morbidity, so antimicrobial regimens are recommended to
reduce long term complications.
AIM :
The study was aimed to detect pyogenic bacteria in clinical pus samples and determine their
antibiotic pattern
METHODS :
The study was conducted in Department of Microbiology, Kakatiya Medical College, Warangal
from July 2021 to December 2021. A total of 525 pus samples were collected. Pus samples were
collected with disposable sterile cotton swab and pus aspirates in syringes under aseptic
precautions, and were transported to microbiology laboratory immediately. The pus samples
were inoculated on Blood agar and Mac conkey aga. The plates were incubated at 370 c for 18-24
hours. Identification of organisms were done by using biochemical reactions. Antibiotic
sensitivity test was done by Kirby Bauer disc diffusion method according to CLSI guidelines.
RESULTS :
Among 525 samples, 275 (52.38%) samples were positive for growth. Gram negative organisms
were isolated more than gram positive organisms. Pseudomonas aeuriginosa was isolated
predominantly among 275 positive samples, 67(24.3%) out of them was only Pseudomonas
aeruginosa, followed by staphylococcus aureus 19.6%, Klebsiella pneumonia 17%, Escherichia
coli 11.2%. In our study Gram negative organisms were more sensitive to Meropenem,
Piperacillin/Tazobactam and Imipenem; Gram positive bacteria were more sensitive to
Vancomycin and Linezolid.