European Journal of Molecular & Clinical Medicine

Abstract

Vitamin D is an important element for skeletal health and it may also affect extraskeletal
health such as association with autoimmune diseases. Inclusive studies in have reported
an association between thyroid autoimmunity and 25-hydroxyvitamin D (25-OHD). Therefore, in
the present study, we examined the relationship between serum TSH levels and vitamin D status
among patients with T2DM. Materials and Methods: A cross-sectional study on 500 Patients
was conducted in the department of Biochemistry, Patna Medical College & Hospital, Patna,
Bihar from January 2021 to December 2021. The study included patients who were diagnosed
with T2DM and were 20 years or older. Exclusion criteria were known hepatic or renal disease,
metabolic bone disease, malabsorption, hypercortisolism, pregnancy and medications influencing
bone metabolism. The serum concentration of 25-OHD was measured by competitive protein
binding assay using kits (Immunodiagnostic, Bensheim, Germany). Glycosylated hemoglobin
(HbA1c) was measured by the high performance liquid chromatography method (Bio-Rad
Laboratories, Waters, MA, USA). TSH levels between 0.22-4.2 mIU/L were regarded normal.
The study was approved by the Institutional Ethical Committee. The statistical analysis was
conducted with SPSS version 23.0 for Windows. Results: A total of 500 participants were
included in this study. Average age of the study population was 47.6 ± 11.2 years (Table 1).
VDD was detected among 52.7% of the study population. Among 8.4%, TSH was lower than
0.22 mIU/L and in 70.2%, TSH was within normal reference range. Abnormally high levels of
TSH (>4.2 mIU/L) were reported in 21.4% subjects. Conclusion: This study suggests positive
associations between the VDD and TSH level among T2DM patients.