European Journal of Molecular & Clinical Medicine

Abstract

Back ground: The "gold standard" for diagnosing an MV infection in a lab is the presence of particular immunoglobulin M (IgM), which can be detected using either an ELISA (enzyme-linked immunosorbent assay)or immunofluorescence. A most popular type of testing is serological because it is quick, affordable, and reliable and can be done in a high-throughput setting. According to earlier research comparing IgM tests, with RT-PCR-based assays as a reference, sensitivities ranged from 89.9 to 98.8% and specificities ranged from 92.5 to 97.9%.
With this background, this study was conducted to serologically diagnose clinically suspected cases of measles by IgM Antibody detection in our setting and also to correlate measles infection with vaccination status.
Materials and Methods: We included all patients aged less than 24 years belonging to both genders visiting the Paediatrics/Medicine OPD at Sir Ronald Ross Institute of Tropical and Communicable diseases, Nallakunta, Hyderabad, during March 2019 to March 2020. The sample size considered was 100. ELISA kit with IgM for measles was used to serologically diagnose measles in suspected cases.