Assessment of bacteriological profile of bladder stones
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 6, Pages 237-241
AbstractBackground: Urinary stone disease (USD) is an increasing clinical problem in both children and adults. The present study was conducted to assess bacteriological profile of bladder stones.
Materials & Methods: 80 patients of urinary stones of both genders were included. Midstream urine specimen was collected from each patient before surgical stone removal and cultured using calibrated (4mm dia.) loop providing fixed quantity on CLED agar, MacConkey agar (MA) and Blood agar plate (BA) (HiMedia Laboratories) for isolation of bacterial pathogens. Cultures were incubated at 37oC for 24 hours. Urinary pH was noted using pH test strip and color change was documented comparing with the provided color chart.
Results: Out of 80 patients, males were 46 and females were 34.UTI was seen in 56 and stone core culture infection in 24. Urine pH was acidic in 52 and alkaline in 28. The difference was significant (P< 0.05). Bacteriological profile in UTI and stone core culture was staphylococcus aureus in 24 and 12, E. coli in 16 and 6, Klebsiella aerogenes in 4 and 3, Pseudomonas aeruginosa in 6, mixed organisms in 4 and 3 and proteus mirabilis in 2 respectively. The difference was significant (P< 0.05).
Conclusion: The most common type of organism associated was E. coli. This study highlights the importance of microbiological analysis of stones for complete sterilization of urinary system and prevention of recurrence.
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