European Journal of Molecular & Clinical Medicine

Abstract

Introduction:Cervical cancer is fourth most common cancer in women and account for about 5% of cancer deaths in females’ worldwide. In India, cervical cancer ranks the first most frequent cancer among females between 15 and 44 years of age. About 99% cervical cancersare linked with Human papilloma virus (HPV).
Aim:To evaluate the immunohistochemical expression of p16/INK4A and MIB-1 in preneoplastic and neoplastic lesions of cervix.
Material and Methods:
The cross sectional study was conducted on 63 cases in department of pathology of our institute over a period of two year from Dec 2018 to Dec 2020. The specimens were processed and three sections of 2-3 micron thickness were made and one stained with H&E stain, examined and 63 cases were selected. Other two sections were used for immunohistochemical staining
Result:In the present study, out of 63 cases, maximum 40 (63.49%) cases were observed in 31-50 years age group. In this study on P16INK4a immunostaining, maximum cases (8/15) of CIN-I and (9/15) of CIN-II histology were found grade-2 and grade-3 positivity respectively. Maximum (2/3) cases of CIN-II and all cases of squamous cell carcinoma histology showed grade-3 positivity. In this study onMIB-1 (Ki67) immunostaining, Maximum (12/15) cases of CIN-I were found grade-1 positive. All cases of CIN-II, III and carcinoma cervixes were positive.
Conclusion: We concluded that p16 INK4A and MIB-1 over expression was observed in CIN I/LSIL, CIN II/HSIL, CIN III/HSIL. Significant up regulation of P16 INK4A and MIB-1 was observed in carcinoma cervix.