European Journal of Molecular & Clinical Medicine

Abstract

Background: Creatinine clearance is a test used to assess renal function(glomerular filtration rate-GFR)) and also for staging chronic kidney disease (CKD) . Urine creatinine estimation requires collection of 24h urine which is a cumbersome and tedious process. Our study proposes a unique fractional urine collection method for urine creatinine estimation that would entail collection of small volumes of urine each time patient voids over 24h period and then compare this new method with conventional 24hour collection method.
Methods: It is a Cross-sectional, prospective study in tertiary care hospital. Volunteers (57) with normal renal function and chronic kidney disease subjects (22) were recruited. Unique method was compared with a conventional method  after centrifugation and with and without preservative. Interclass correlation coefficient (ICC) and Bland Altman analysis was used to evaluate for agreement between the two methods.
Results: All the values were combined without categorization into the subgroups. Creatinine results of fractional urine collection method without preservative (UF1) and with preservative (UF2) was compared to conventional method (U24), ICC was 0.86 (C.I, 0.80 to 0.90) and 0.84 (C.I, 0.77 to 0.89) respectively.
Conclusions: The urine creatinine results of unique collection method is comparable and reliable as traditional 24-hour method