Abstract

Introduction: A key diagnostic material for aerobic growth and susceptibility testing in the microbiology laboratory is pus. It is crucial to periodically conduct investigations to ascertain the evaluate the safety of microorganisms since the rate of emergence of resistance in bacterial isolates considerably outpaces the frequency of subsequent drug discovery and development.
Aim: This investigation was done to assess the evolving pattern of antibiotic resistance in different pus specimens.
Materials and Methods: Pus specimen was obtainedfrom several Department of Microbiology, MGM Medical college n Lion’s Seva Kendra, Kishanganjover the course of six months using an aseptic approach, and they were processed right away in the lab using conventional antimicrobial techniques. Motility tests, Gram staining, and biochemical processes were used to microbes. All specimens underwent Kirby Bauer's disc diffusion method antibiotics susceptibility testing on Muller Hinton agar, with results interpreted in accordance with CLSI recommendations.
Results: 120 pus specimens were analysed, and the results revealed that surgeries departments (32.42%) contributed the most to the 93.26% culture positive. One of most prevalent organism was Pseudomonas, followed by Staphylococcus aureus (22.51%). Gram negative bacilli were responsive to Imipenem (87.09%), Piperacillin (61.28%), and Gentamicin (48.38%) while Gram positive cocci were susceptible to Linezolid (94.86%), Vancomycin (92.30%), and Imipenem (92.30%).
Conclusions: As per regions and growing multi-resistant bacteria, the shifting patterns of antibiotic sensitivity in isolated strains from pus might be a useful tool for doctors to begin empiric therapy of patients as soon as possible