Effect of herbal solutions on antimicrobial efficacy of triple antibiotics when used as delivery vehicle: An in-vitro study
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 7, Pages 5710-5717
AbstractIntroduction: Microbes elimination in the root canal system is endodontic therapy's prime goal. It is advised to utilise intracanal medicaments between the appointments to diminish bacterial counts. This study was conducted to investigate the effect of herbal solutions on antimicrobial efficacy of triple Antibiotics when used as delivery vehicle for intracanal medicament.
Methodology: 160 extracted single-rooted teeth were manually instrumented. After contaminating the canals with E. Faecalis and C. albicans, the specimens were divided randomly into 4 equal groups (n 20) for each C. albicans and Faecalis according to the intra-canal medications used: Group I: Clove Oil, Group II: Neem oil, Group III: Cinnamon oil, Group IV: Saline, All the three Groups were mixed with triple Antibiotics Ciprofloxacin, Metronidazole and Doxycycline. Microbial samples were obtained from the root canals after 24 hours, and cultures were determined after 24 hours of incubation. The Colony Forming unit was analysed with Kruskal- Wallis, Dunn's, and Chi-Square tests to find significant differences among different groups.
Results: The number of colony-forming units was significantly lower in all experimental groups compared to the control group in both agars, the highest mean rank for colony count in E. Faecalis was 34.1 and in Candida Albicans was 29.30. Neem oil had better antimicrobial efficacy compared to other medications when it was mixed with Triple antibiotics.
Conclusions: The study suggests that Neem oil increased the efficacy of triple antibiotics to eliminate the two different tested microorganisms at the root canal lumen. C.albicans was the most sensitive tested microorganism to the whole tested medications
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