European Journal of Molecular & Clinical Medicine

Abstract

BACKGROUND :-
Colon cancer is known to be the third most deadly and fourth most commonly diagnosed cancer in the world. In this research we study the anti-cancer properties of the plant leaf extract of Tecoma stans. There has always been a need for anti-cancer agents, since the commercially available synthetic drugs have their own limitations due to undesirable side effects. AIM :- To analyse the antioxidant and cytotoxic activity of Hydroethanolic leaf extract of Tecoma stans in colon cancer cell lines.
 
MATERIALS AND METHODS :-
Human colon cancer cell line (HT -29) was brought from NCCS, Pune, India. Cell viability test and Gene expression analysis were carried out for P-I3 and Akt mRNA Gene expression using MTT and PCR respectively. The results were analyzed using appropriate statistical tools.
RESULT :-
Results suggest that maximum reduction of cell proliferation was at dosage of (100-500 μg/ml) of the Hydro ethanolic leaf extract of Tecoma stans used in the study when compared to untreated group. At doses of 300 g/ml and 400 g/ml, the cancer cells were severely suppressed and mRNA expression of P-I3 and Akt was significantly reduced as compared to control.
 
CONCLUSION :-
The research carried out revealed that leaf extracts of Tecoma stans have diverse pharmacological spectrum and contain anti-cancer behavious. Research on this plant is increasing day by day because of its potent pharmacological uses. However further investigations involving the isolation on screening models of constituents are needed for further confirmation of various potential of Tecoma stans