European Journal of Molecular & Clinical Medicine

Abstract

Background :   Vigorous growth of cells leads to cancer. Tecoma Stans, a flower which consists of anti - cancer properties. One of such anti - cancer properties is the pro - apoptotic role in melanoma cell line (A-375). This is an in vitro study. The intervention is a hydroethanolic extract of Tecomastans on the melanoma cell line. The gene expressions chosen for this study are P53, caspase 3 and caspase 9. Aim of this study is to assess the pro apoptotic role of Tecomastans in melanoma.
 
Materials and methods : Human skin cancer (A - 375) was brought from NCCS, Pune, India. Cell viability test using MTT assay and Gene expression analysis for P53, caspase 3 and caspase 9 were carried out using MTT and PCR respectively. The results were analyzed using appropriate statistical tools using ANOVA and Duncan’s  test.
 
Results : The study suggestin MTT assay, the percentage of cell viability is observed to decrease on induction of Tecoma Stans.Caspase 9, mRNA  gene expression decreases on induction of dosages 300mg/ml and 400mg/ml of Tecomastans. P53 gene expression increases on induction of dosages 300mg/ml and 400umg/ml of Tecomastans. Caspase 3 gene expression doesn’t show any significance on induction of dosages 300mg/ml and 400mg/ml of Tecomastans.  
 Conclusion : Tecomastans consists of pro apoptotic property which is one of the anti cancer properties in melanoma when acted on the gene expressions P53, caspase 3 and caspase 9.