Isolation of bacteria from pus samples and antibiotic sensitivity pattern in a tertiary care hospital, Lucknow
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 7, Pages 8492-8497
AbstractIntroduction: In hospitals pyogenic infection is one of the major cause of morbidity. Increasing multidrug resistant strains has made treatment of such infection difficult. For correct antibiotic use, every pus samples should undergo culture and sensitivity.
Aim:Toisolatebacteriafrom pus samples and antibiotic sensitivity pattern in various pus isolates.
i) To study the distribution pattern and prevelance of bacteria causing pyogenic infection.
ii) TostudyAntibioticSensitivity Pattern of organism isolated from pus.
Methods: The study was conducted in the Department of Microbiology, T.S. Misra Medical College & Hospital, Lucknow, for a duration of 6 months. 256 pus samples from various wards with suspected pyogenic infection were studied. Bacteria were isolated, identified and antibiotic profile was determined from pus samples using standard protocol.
Results: In our study, out of 256 pus samples studied, 159 (62.10%) samples were positive for growth. Gram positive bacteria outnumbered gram negative isolates. Commonest isolate was Staphylococcus aureus, followed by Coagulase Negative Staphylococcus and Pseudomonas aeruginosa. All Staphylococcus aureus were sensitive to Vancomycin and Linezolid. All Pseudomonas aeruginosa were sensitive to Colistin. In Enterobacteriaceae group most effective antibiotic were Colistin and Tigecyclin.
Conclusion: It is important for a clinician to send all the pus samples for microbiological analysis and their antibiogram before putting cases on antibiotic so that emergence of drug resistance can be minimized.
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