European Journal of Molecular & Clinical Medicine

Abstract

A simple, accurate and precise high-performance thin layer chromatographic method for simultaneous estimation of Salicylic acid (SA) and Lactic acid (LA) in their combined liquid dosage form has been developed. The method employed thin layer chromatographic aluminium plates pre-coated with silica gel 60 F₂₅₄ as the stationary phase and chloroform: formic acid: methanol (8.5: 1:0.5 v/v/v) as mobile phase. Chromatographic analysis was carried out in the reflectance/absorbance mode at 229 nm. The method was validated with respect to linearity, specificity, accuracy, precision, limit of detection and limit of quantitation and applied for analysis of SA and LA in combined dosage form. The Rf values were found to be 0.285 ± 1.922 and 0.688 ± 1.094 for SA and LA, respectively. The linear regression analysis data for the calibration plots showed a linear relationship in the concentration range 200-1200 ng/band with correlation coefficient 0.9983 for SA and 200-1200 ng/band with correlation coefficient 0.998 for LA. The proposed method can be applied for routine analysis of dosage form containing SA and LA in combination.