ANTI METASTATIC EFFECT OF PERGULARIA DAEMIA ON BREAST (MCF-7) CANCER CELL LINE
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 8, Pages 2601-2608
AbstractBackground: Breast cancer develops as a result of genetic mutations or damage to DNA. On average only 35% women with advanced breast cancer are alive five year after diagnosis. However, search for new anticancer drugs for effective treatment is on as the plant- based system of medicine does not pose serious problems. So, the aim of this study is to analyze the antimetastatic effect of Pergularia daemia on Breast (MCF-7) cancer cell lines
Materials and Methods: P.daemia was collected, powdered and Soxhlet extracted with 70% ethanol. It was then filtered and solvent evaporated to get a viscous mass, which was then stored at 4∘C until used.
Results: By optimizing the concentration of treated P.daemia it significantly decreases cell viability. Bcl-2 and Bcl-xL show significant decrease in expression with higher concentration of P.daemia treated cells. A significant increase in the Caspase-3 mRNA activity was observed after incubation with the leaf extract of P.daemia.
Conclusion: Pergularia daemia exhibits antimetastatic effect on breast (MCF-7) cancer cell lines. The active component of P.daemia may promote the antimetastatic effect on breast cancer cell lines.
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