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Analytical method development and validation for assay method of Piribedil and Indapamide by High Performance Liquid Chromatography

    Author

    • Vinod D. Godse, Priyadarshani R. Kamble, Aejaz Ahmed
,

Document Type : Research Article

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Abstract

A reverse phase liquid chromatography method (RP-HPLC) was developed to estimate the amount of Piribedil and Indapamide in bulk and its pharmaceutical formulations. Agilent HPLC system equipped with auto sampler, UV-Visible detector Nucleosil 5 C18, 15 cm X 4.6 mm, 5 µm was used for chromatographic separation by using at a detection wavelength of 233 nm was used. Keeping the flow rate of 1.3 mL/min the composition of mobile phase water: acetonitrile: methanol: trifluro acetic acid (80:18:02:0.2) mL/v: v: v: v in given amount were used for the quantification of the drugs. The objective of this study was to developed and validated for the routine analysis of Piribedil and Indapamide in API and tablet dosage forms. The developed method was validated according to the ICH guidelines. The linearity, precision, range, and robustness were within the limits as specified by the ICH guidelines. Hence, the method was found to be simple, accurate, precise, economic, and reproducible

Keywords

  • Indapamide
  • Piribedil
  • HPLC
  • Assay method
  • ICH guidelin
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    • Article View: 44
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European Journal of Molecular & Clinical Medicine
Volume 9, Issue 6
August 2022
Page 2587-2601
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  • Article View: 44
  • PDF Download: 95

APA

Aejaz Ahmed, V. D. G. P. R. K. (2023). Analytical method development and validation for assay method of Piribedil and Indapamide by High Performance Liquid Chromatography. European Journal of Molecular & Clinical Medicine, 9(6), 2587-2601.

MLA

Vinod D. Godse, Priyadarshani R. Kamble, Aejaz Ahmed. "Analytical method development and validation for assay method of Piribedil and Indapamide by High Performance Liquid Chromatography". European Journal of Molecular & Clinical Medicine, 9, 6, 2023, 2587-2601.

HARVARD

Aejaz Ahmed, V. D. G. P. R. K. (2023). 'Analytical method development and validation for assay method of Piribedil and Indapamide by High Performance Liquid Chromatography', European Journal of Molecular & Clinical Medicine, 9(6), pp. 2587-2601.

VANCOUVER

Aejaz Ahmed, V. D. G. P. R. K. Analytical method development and validation for assay method of Piribedil and Indapamide by High Performance Liquid Chromatography. European Journal of Molecular & Clinical Medicine, 2023; 9(6): 2587-2601.

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