Histological and histochemical study on the structural development of the small intestine in cape hare Lepus capensis
European Journal of Molecular & Clinical Medicine,
2023, Volume 10, Issue 3, Pages 860-869
AbstractThis study's objective was to investigate how the small intestine develops in cape hares by the histological and histochemical techniques. The investigation was conducted on (15) samples of the small intestine, five at the age of one day, five at the age of fourteen days, and five at the age of adulthood, shortly following the slaughter, samples were collected from various regions of (duodenum, jejunum, and ileum). Ten samples from each area were collected and fixed in 10% formalin for 24 hours before being subjected to standard histological procedures and having the sections stained with H&E, PAS, AB, PAS-AB, and Masson's Trichrome to identify the various components. The current study revealed a large number of vacuolated enterocytes and immature cells in one-day-old pups, but no Peyer's patches, Brunner's gland, or Crypts of Lieberkuhn were discovered. Instead, there were clumps of immature epithelial cells with large and pale nucleus near the bases of the villi. The vacuolated cells vanished in both the 14-day-old and adult forms, and the villi took on their mature morphology with wide bases and tiny points. In the duodenum, the villi were fashioned like leaves, while those in the jejunum and ileum were conical. Simple columnar epithelium with many goblet cells lined the mucosa in all the segments of small intestine, and the goblet cells found in all ages, Crypts were present firstly in 14 day age. In 14 day and adult The Brunner's glands in the first part of the duodenum were of the serous type. At the end of the adult hare ileum was Peyer's patch. All ages had the sacculus rotundus dispersed with multiple lymph nodule that contributed to immunological response in the gut, nerve plexuses in each of submucosa and muscularis found in all ages.
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