European Journal of Molecular & Clinical Medicine

Abstract

Background: Hypothyroidism is a condition caused due to decreased synthesis, metabolism or
decreased action of thyroid hormone which can be due to various causes. Primary
hypothyroidism is due to conditions affecting the thyroid gland itself. It accounts for
approximately 99% of cases, with < 1% being due to TSH deficiency known as central or
secondary hypothyroidism Objectives: To clinically suspect and diagnose cases of
hypothyroidism.To measure serum ferritin levels in newly diagnosed hypothyroid patients.To
look for a correlation between serum ferritin level and T3, T4 and TSH levels.Methodology:
30 patients with clinically suspected hypothyroidism were confirmed by measuring fasting
serum T3, T4 and TSH levels.Complete Blood Picture, Liver Function Tests, Renal Function
Tests and Urine Pregnancy Test done to rule out other causes that could alter thyroid function
test.After an overnight fasting of 8 hours, 2ml of venous sample was collected in a red cap
vacutainer under aseptic conditions and serum T3, T4 and TSH levels were measured using
chemiluminescence immunoassay (CLIA) .In patients with confirmed hypothyroidism, serum
ferritin levels were estimated.For this, 2ml of venous blood sample was collected in a red cap
European Journal of Molecular & Clinical Medicine
ISSN2515-8260 Volume10, Issue 04,2023
179
vacutainer under aseptic conditions and serum ferritin levels was measured using CLIA
Results:The mean serum T3,T4 and TSH levels were 0.5203 ng/ml,3.50 ug/dl,40.02 uIU/ml
respectively.The overall mean serum ferritin was 34.24 ng/ml.The mean serum ferritin among
patients in whom correlation was seen was 8.2 ng/ml.The mean serum ferritin among patients
in whom correlation was not seen was 45 ng/ml.A significant negative correlation of 0.01 was
found between serum ferritin and TSH levels indicating that patients with lower serum ferritin
had higher serum TSH levels.Conclusion :Patients presented with weight gain and fatigue,
serum ferritin had a significant negative correlation with TSH indicating that lower ferritin
levels were associated with higher TSH levels.