European Journal of Molecular & Clinical Medicine

Abstract

Background:Experimental studies have confirmed the probability of
changes in some carbohydrate and protein metabolism indicators during
dichloromethane intoxication under the conditions of noise and vibration. It is
necessary to use Phytotea’ Gepalyuks’ with potassium for prevention and
treatment by correcting metabolic processes to avoid the adverse effects of
physical and chemical factors on the body.
Objective: Experimental study of the effectiveness of phytotea
“Gepalyuks” and potassium on carbohydrate energy metabolism of the liver and
blood under the influence of chemical and physical factors.
Methods: The experiments were conducted on 98 purebred white rats -
males weighing 160-180 years, contained in vivarium’s usual diet. Animals were
divided into control and experimental groups. Control animals received an
equivalent volume of drinking water, food and were kept in vivarium conditions.
Animals of the experimental group were divided into two groups: The first group
was intragastrically injected 10% of oiled dichloromethane solution in the dose of
1/20 LD50 (255 mg/kg) in conditions of noise (tape recording) at the level of 95-
105 dBA and vibration of 5-16 dBA during 90 days.
with intragastric administration of 10% oily solution of dichloromethane in a dose
of 1/20 LD50 (255 mg/kg), was administered a decoction of phytotea
“Gepalyuks”(1 ml of phytotea per 100 g of animal body weight) in conditions of
noise exposure (tape recording) at the level of 95-105 dBA and vibration of 5-16
dBA during 90 days. Phytotea “Gepalyuks” consists of immortelle sand flower,
yarrow herb, licorice root, peppermint leaves, corn snouts, and rosehip fruit.
Animals were slaughtered on 15, 30, 60 and 90 days from the beginning of the
experience. We took the liver, blood and determined biochemical indicators of
carbohydrate, protein, and pigment metabolism for research.
We studied some carbohydrate energy metabolism indicators to study the
effectiveness of phytotea“Gepalyuks” and potassium orotate under chronic
exposure to dichloromethane, noise and vibration.
Findings: When administered within 90 days, biologically active vegetable
and synthetic preparations in the form of decoction lead to an increase in the
activity of GDG, SDH and MDH and a decrease in the level of pyruvic and lactic
acids in serum and liver.
As a result of experimental studies, it was found that when administered
phytotea “Hepallux” in combination with potassium orotate for 90 days (15, 30,
60 and 90 days) under chronic exposure to dichloroethane, noise and vibration in
biochemical indicators - protein and lipid metabolism and activity of enzymes of
aliphatic amino acids remineralization (AST, ALT) and the content of cholesterol,
total and direct bilirubin in the liver and blood revealed a positive effect.
Conclusions: Application of phytotea “Hepallux” in complex
Potassium orotate causes a pronounced pharmacotherapeutic effect under
the toxic effects of chemical and physical factors - dichloroethane, noise and
vibration. Normalizes or approximates indices of carbohydrate energy (pyruvic,
lactic acids, the activity of GDG, SDG, MDG) metabolism and alkaline
phosphatase to those of the control group in blood and liver. The content of totalprotein, total and free bilirubins, the activity of AST, ALT in serum and liver is
approaching the norm.