Document Type : Research Article
Abstract
Objective: The current research aimed to evaluate the concentration level of
IL-5 in irritable bowel syndrome patients infected with Blastocystis infection
Methods: Stool samples were collected from 156 of respondents(78 patients
were diagnosed clinically by physician suffering from IBS and 78 control
apparently healthy individuals without any history of disease) attended to
three Hospitals in Al-Najaf Province of Iraq from August, to December
2019. Data was collected using self-administrated questionnaire. The whole
questionnaire has five sections which included age, sex , family income,
living area and occupation.Fecal specimens were collected and each sample
divided into three parts, the first for microscopic examination and second
for culture and thrid part were quickly frozen for Immunological evaluation
of IL-3 by Sandwich ELISA , shipped on dry ice and kept at–20 ºCprior to
analysis.
Results: the Blastocystis was significantly predominant (P>0.001) parasite
associated with IBS which constituted (46.2%). The results of culture were
higher than light microscopic examination (48.7%).
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