Anticoagulant Activity of Ethanolic Leaf Extract of Pithecellobium Dulce Benth. (Fabaceae) in Alloxan-Induced Diabetic Rats
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 2, Pages 4069-4076
AbstractDiabetes is associated with the changes in thrombotic and fibrinolytic coagulation factor level/activity that increases the risk of thrombus formation. Patients with diabetes mellitus (DM) have a higher risk of developing blood clots in the arteries that may lead to a series of complications associated, specifically, with the cardiovascular system. Blood clots in the arteries of the heart accounts for 80% mortality rate of diabetic patients. In this study, the researchers evaluated the anticoagulant effect of Pithecellobium dulce in diabetes and its roles in the hypercoagulation. Leaf extracts of P. dulce were orally administered to alloxan-induced diabetic male sprague-dawley rats for seven consecutive days. The induction of diabetes was described through glucose testing which was measured prior to the start of drug administration. The rats were divided into six groups consisting of six rats each. Normal saline solution and warfarin 3 mg/kg BW were administered as the negative and the positive controls, respectively. Doses of 200 mg/kg, 400 mg/kg, and 600 mg/kg BW were administered once daily for seven days. Samples of blood were drawn after seven days of treatment and were centrifuged at 5000 rpm for 15 minutes. Anticoagulant activity was measured using prothrombin time (PT), activated partial thromboplastin time (aPPT), thrombin time (TT). In conclusion, P. dulce did not exhibit anticoagulant effect as demonstrated by statistical significance by shortened period for clot formation compared to the positive control.
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