• Register
  • Login

European Journal of Molecular & Clinical Medicine

  1. Home
  2. In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines

    Authors

    • Ayyub A. Patel 1
    • Mohammed Amanullah 1
    • Fahmy G. Elsaid 2
    • Tamer Soliman 3
    • Mohamed Eissa 4
    • Hani Alothaid 5

    1 Clinical Biochemistry Department, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia

    2 Biology Department, College of Science, King Khalid University, Abha, Kingdom of Saudi Arabia; Zoology Department, Faculty of Science. Mansoura University, Mansoura, Egypt

    3 Clinical Pathology Department, Faculty of Medicine, Sohag University, Sohag, Egypt

    4 Pathology Department, College of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia; Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    5 Department of Basic Medical Sciences, Faculty of Applied Medical Sciences, Al-Baha University, Al-Baha, Saudi Arabia

,

Document Type : Research Article

  • Article Information
  • Download
  • Export Citation
  • Statistics
  • Share

Abstract

For centuries, plants are known to play part in the daily routine from providing food to the management of human health. During the last few decades, diverse phytochemicals have been characterized as agents that possess a potential to execute cancer cells. The oil extracted from the root of Saussurea costus; has been used in traditional medicine for a long time. Purpose: This study was carried out to assess the effect of Saussurea lappa aqueous extract on five different cell lines; Human Lung Fibroblast cells (MRC5), Human Dermal Fibroblast adult (HDFa), Madin-Darby Canine Kidney cells (MDCK), breast cancer cells (MCF7) and human colonic cancer (Caco2). Results: On normal cell lines; the extract showed cytotoxic activities with the half maximal inhibitory concentration; IC50 values, ranging from 0.85 to 2.5 mg/ml whereas MRC5 was the most sensitive cell line (IC50 values: 0.85). On malignant cell lines; MCF7 cells showed inhibition in cell proliferation with activity from 67.17% to 91.01% by the extract treatment concentration 0.50 to 2.0 mg/dl. However, Caco2 cells exhibited inhibition in proliferation ranging from 37.10% to 69.45% by the same treatment dose. Thus, breast cancer cells were more sensitive than colon cancer cells to Saussurea costus extract. The genotoxic potentialities of the crude extract showed increase in expression of proapoptotic genes; P53, IκBα, BAX and TNF and decrease in the expression of antiapoptotic genes; Bcl2, Survivin, and MMP-7. Conclusion: Our work pointed out that Saussurea lappa extract could be a potential candidate that has the capability to fight cancer.

Keywords

  • Saussurea lappa
  • colon cancer
  • breast cancer
  • Dermal fibroblasts
  • Genotoxic potentialities
  • XML
  • PDF 653.81 K
  • RIS
  • EndNote
  • Mendeley
  • BibTeX
  • APA
  • MLA
  • HARVARD
  • VANCOUVER
    • Article View: 398
    • PDF Download: 438
European Journal of Molecular & Clinical Medicine
Volume 7, Issue 9
December 2020
Page 736-747
Files
  • XML
  • PDF 653.81 K
Share
Export Citation
  • RIS
  • EndNote
  • Mendeley
  • BibTeX
  • APA
  • MLA
  • HARVARD
  • VANCOUVER
Statistics
  • Article View: 398
  • PDF Download: 438

APA

Patel, A. A., Amanullah, M., Elsaid, F. G., Soliman, T., Eissa, M., & Alothaid, H. (2020). In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines. European Journal of Molecular & Clinical Medicine, 7(9), 736-747.

MLA

Ayyub A. Patel; Mohammed Amanullah; Fahmy G. Elsaid; Tamer Soliman; Mohamed Eissa; Hani Alothaid. "In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines". European Journal of Molecular & Clinical Medicine, 7, 9, 2020, 736-747.

HARVARD

Patel, A. A., Amanullah, M., Elsaid, F. G., Soliman, T., Eissa, M., Alothaid, H. (2020). 'In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines', European Journal of Molecular & Clinical Medicine, 7(9), pp. 736-747.

VANCOUVER

Patel, A. A., Amanullah, M., Elsaid, F. G., Soliman, T., Eissa, M., Alothaid, H. In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines. European Journal of Molecular & Clinical Medicine, 2020; 7(9): 736-747.

  • Home
  • About Journal
  • Editorial Board
  • Submit Manuscript
  • Contact Us
  • Glossary
  • Sitemap

News

 

For Special Issue Proposal : editor.ejmcm21@gmail.com

Newsletter Subscription

Subscribe to the journal newsletter and receive the latest news and updates

© Journal Management System. Powered by ejournalplus