European Journal of Molecular & Clinical Medicine

Abstract

A conductometric titration method has been developed for determination of free organic acids in anise fruits. The direct acid base titration of free organic acids was carried as a titrant was used the standardized sodium hydroxide aqueous solution. The end-point of titration was fixed by a conductometric electrode. The calibration curve was found to be linear in the concentration range of 0.0112 - 0.0332 mg/cm3 (r2 = 0.9992). The percentage recovery was found to be in the range of 98.61 ‐ 101.25%. The intra-day and inter-day precision were 1.32% and 1.62%, respectively. The method was found to be accurate and precise, having relative standard deviation of less than 2%. The developed method was validated statistically by compliance with SPhU guidelines. The proposed method is simple, convenient, rapid and sufficiently precise and can be successfully applied for the quantitative determination of total free organic acids in anise fruits.