MOLECULAR GENETIC MECHANISMS OF HCV INFECTION CHRONICITY
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 10, Pages 407-412
AbstractResearch objective: to analyze the distribution of alleles and genotypes frequencies and to assess the predictive significance of the polymorphism of the TNF-α, CTLA-4, CYP2E1, CYP2C9 *2, CYP2C9 *3, CYP3A4 and CYP1A2 genes of cytochrome P450 in Uzbek patients with chronic hepatitis C and liver cirrhosis.
Material and methods. The study included the analysis of a core group, consisting of 107 patients with chronic hepatitis C (CVHC) and the control of 81 conditionally healthy individuals. All surveyed patients were of Uzbek nationality, with a verified diagnosis of chronic hepatitis C and liver cirrhosis. DNA extraction from lymphocyte nuclei was carried out according to the Sambrook J. et al method. SNP-genotyping of gene polymorphisms was carried out by standard PCR on soft thermal cyclers "Corbett Research" (Australia) and "Applied Biosystems" (USA).
Results. The research showed that the predictive efficacy of each of the genetic polymorphisms in the core group was relatively low (AUC value was from 0.51 to 0.57). Besides, the revealed features of the prevalence of allele frequencies and the genotypes distribution of the studied cytochrome P450 isoenzymes were a significant factor in determination of the CVHC pathogenesis and the disease course. The results indicated a link between the expression of CYP2E1, CYP2C9, CYP1A2 and disease progression.
Conclusion. According to the marker incidence, CTLA-4 -49A/G gene polymorphism has a good predictive efficiency in relation to the development and course of CVHC.
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