Cost Effective Salivary Viral DNA Isolation in Limited Resource Setting – A Pilot study
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 10, Pages 703-707
AbstractBackground: The various diagnostic procedures that requires isolation of viral products from serum, saliva and other body fluids involves cumbersome extraction kits. These kits are very expensive and involve highly technique sensitive procedures. Aim: This pilot study involves systematic review and exploring an option of utilizing the available laboratory materials to perform viral DNA isolation and ensuring the best quality of the isolated DNA through the agar-gel electrophoresis followed by image recording. Materials and methods: Deep throat gargle followed by vigorous rinsing of oral cavity done after mild scrapping of posterior tongue and anterior faucial pillars using ice cream stick in order to ensure enough squamous cell collection. The collected Oral rinse was stored in -80 till the DNA isolation procedure.The isolated DNA was captured through Agar-gel electrophoresis and imaged through special analyzer. Conclusion: Equivalent quality of DNA as well as RNA were isolated through this comparativelycost effective method.
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