Comparison Between The Effects Of Two Recaldent® Products And Artificial Saliva On The Hardness Of Enamel Exposed And Not Exposed To A Carbonated Beverage – An In Vitro Study
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 8, Pages 1898-1907
AbstractThis in vitro experimental study on 48 extracted sound human maxillary central incisors was conducted to evaluate the effects of two Recaldent® products and artificial saliva on the hardness of enamel not exposed to and exposed to a carbonated beverage. Coca-cola was the test beverage. All hardness measurements were taken with Vickers hardness tester.
Three groups of samples (groups B1,B2 and B3) were subjected to the erosion process with the carbonated beverage. Groups A1, A2 and A3 were not subjected to the erosion process. For the remineralisation process, a thin layer of test material I (CPP-ACFP) was applied on the enamel surfaces of the samples in groups A1 and B1 for three minutes and then stored in artificial saliva for 6 hours.
A two stage blinding procedure was performed. Data was entered into MS Excel spreadsheet and processed using SPSS software version 15.0. One way analysis of variance (ANOVA), Student t test, Post Hoc ( Tukey ) test and Bonferroni test were performed.
Results showed that differences between the sub groups at baseline was statistically not significant. The difference in enamel microhardness between baseline and demineralization in the groups A1, A2, A3, B1, B2, B3 were 1.1, 0.4, 1.1, ―44.4, ―44.5 and ―46.3 respectively. The mean difference in enamel microhardness measured after demineralisation and after remineralisationin the groups A1, A2, A3, B1, B2, B3 were 3.8, 2.9, 0, 14.3, 13.2, 2.7 respectively. Post Hoc (Tukey test) test performed between the groups B1 and B2 after remineralisation did not show statistically significant difference between the groups (p = 0.888).
Through this in vitro study, it can be concluded that carbonated beverages decrease the microhardness of tooth enamel. CPP-ACP and CPP-ACFP increase the microhardness of enamel. Artificial saliva increases the hardness of enamel, but to a lesser extent than CPP-ACP and CPP-ACFP. There is no difference between the remineralising capacity of CPP-ACP and CPP-ACFP.
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