Prospective Study Of Isolates In Pyogenic Samples
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 7, Pages 4052-4062
AbstractThe advancement of specific automated systems in microbiology lab has increased the prognosis of treatment for various microbial diseases that were uncured during past. Various automated systems like VITEK 2, microscan make investigation criteria in simple and easy way such that microorganism specific for there biochemical tests are observed under computer operated device which makes the reporting quick and unbiased. In our medical center, we have diagnosed many patients with pyogenic infection. Our aim was to study the isolates that were isolated from consecutive pyogenic samples coming in the hospital. We performed a prospective review of the records of the 70 unselected patients coming with pyogenic infection. We proceed the samples manually along with automated systems. Initial staining and culturing was done on BA, CA, MA which were later observed twice after 24hrs and 48hrs. There was significant change in positivity of sample in case of difference in age and gender. According to the observations carried out by us that percentage of positivity increase with increase of age and with every male gender. Staphylococcus aureus was the the majorly identified organism in study of consecutive pyogenic samples. Various anti toxins, enzymes, anti immune strategies of this organism were studied that are helpful for this bug to cause disease. The evaluations also included an assessment of risk factors, antimicrobial therapy and manual diagnosis methods. By Universal infection-control measures, the transmission of infections may be limited, patient education, screening and decolonization.
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