European Journal of Molecular & Clinical Medicine

Abstract

This study aimed at developing a new technique of vasectomy, loupe-assisted vasovasostomy and tissue sampling hoping to achieve better results for experimental studies using a rabbit model. The technique described is typically helpful for anatomists undergoing similar research on animal model. The total number of rabbits used in this study was twenty-nine male rabbits of white New Zealand breeds. The animals were anaesthetized and bilateral vasectomy was carried out by ligation of the vas deferens at two points. The ligatures were put 1 cm apart. The suture material used was 4-0 nylon non- absorbable suture material. The segment between the two ligatures was excised and utmost care was observed to avoid damage to the blood vessels or sutures. Bilateral vasovasostomy was carried out after 16 weeks on 21 of the vasectomized animals (8 rabbits acted as control). One-layer method was adopted, using low power magnification spectacles (x 5) and non-absorbable suture material. Samples were obtained from vasovasostomized animals, under general anesthesia after, 24, 28, 32 and 36 weeks from the beginning of the experiment. The overall operation technique of loupe assisted modified one-layer technique was satisfactory and mean operation time was 70 - 100 minutes. The described technique is so clear that it could be adopted by other researchers intending to do similar work on vasectomy or vasovasostomy.