• Register
  • Login

European Journal of Molecular & Clinical Medicine

  • Home
  • Browse
    • Current Issue
    • By Issue
    • By Subject
    • Keyword Index
    • Author Index
    • Indexing Databases XML
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Contact Us
Advanced Search

Notice

As part of Open Journals’ initiatives, we create website for scholarly open access journals. If you are responsible for this journal and would like to know more about how to use the editorial system, please visit our website at https://ejournalplus.com or
send us an email to info@ejournalplus.com

We will contact you soon

  1. Home
  2. Volume 7, Issue 11
  3. Author

Online ISSN: 2515-8260

Volume7, Issue11

Validated RP-HPLC Method Development for the Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Combined Dosage Form

    Prashant D. Ghode, Abhishek R. Kalamkar, Atul S. Sayare, Asawari D. Pachauri, Anil N. Tankar, Nivrutti A Yewale, Shweta P Ghode

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 2576-2585

  • Show Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract

A simple, sensitive, reproducible, accurate and precise RP-HPLC method was developed for simultaneous estimation of Irbesartan and Hydrochlorothiazide in tablet dosage form. The chromatographic separation was achieved on Quails 5 BDS C18 column (250 x 4.6mm, particle size 5µ) in low pressure gradient mode with mobile phase Acetonitrile: water (pH adjusted to 3.3 with orthophosphoric acid)in the ratio (42:58 v/v). The flow rate and injection volume were 1.1ml/min and 10 µL respectively and monitored on a PDA detector at 254nm. The developed method was found to be linear for Irbesartan and hydrochlorothiazide in the range of 12-84µg/ml and 1-7µg/ml with correlation coefficient (r2) 0.9997 and 0.9992 respectively. The proposed method was validated as per ICH Q2B guidelines. Assay of the marketed formulation was found to be 98.26% and 98.47% for Irbesartan and hydrochlorothiazide respectively. In accuracy study the percentage recovery at three different levels was found to be in the range of 98.80% to 101.78%.
Keywords:
  • PDF (360 K)
  • XML
(2021). Validated RP-HPLC Method Development for the Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Combined Dosage Form. European Journal of Molecular & Clinical Medicine, 7(11), 2576-2585.
Prashant D. Ghode, Abhishek R. Kalamkar, Atul S. Sayare, Asawari D. Pachauri, Anil N. Tankar, Nivrutti A Yewale, Shweta P Ghode. "Validated RP-HPLC Method Development for the Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Combined Dosage Form". European Journal of Molecular & Clinical Medicine, 7, 11, 2021, 2576-2585.
(2021). 'Validated RP-HPLC Method Development for the Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Combined Dosage Form', European Journal of Molecular & Clinical Medicine, 7(11), pp. 2576-2585.
Validated RP-HPLC Method Development for the Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Combined Dosage Form. European Journal of Molecular & Clinical Medicine, 2021; 7(11): 2576-2585.
  • RIS
  • EndNote
  • BibTeX
  • APA
  • MLA
  • Harvard
  • Vancouver
  • Article View: 55
  • PDF Download: 91
  • LinkedIn
  • Twitter
  • Facebook
  • Google
  • Telegram
Journal Information

Publisher:

Email:  info@ejmcm.com

  • Home
  • Glossary
  • News
  • Aims and Scope
  • Privacy Policy
  • Sitemap

Editorial Team:  editor@ejmcm.com

For Special Issue Proposal : chiefeditor.ejmcm@gmail.com / info@ejmcm.com

This journal is licensed under a Creative Commons Attribution 4.0 International (CC-BY 4.0)

Powered by eJournalPlus