European Journal of Molecular & Clinical Medicine

Abstract

From the aboveground and root parts of Rumex confertus plants, the method
of triple ethanol extraction was used to extract the polyphenol tannic compounds extract -
ERc and evaluate its biological activity. It was established that ERc with a single oral
administration at a dose of 5000 mg/kg does not cause toxic effects in experimental
animals and their death, with prolonged oral administration at a dose of up to 500 mg/kg
also does not cause animal death and pathological changes in their organs. At
concentrations of 50-100 μg/ml, it inhibited the growth of Bacillus subtilis, Pseudomonas
aeruginosa and Staphylococcus aureu. At a concentration above 100 μg/ml, it showed a
cytotoxic effect on normal fibroblast cells, and at a concentration of 50 μg/ml ERc
significantly stimulated the proliferation of these skin cells, which allowed us to choose
this concentration as effective and introduce it into a collagen based RP. As a result, the
obtained RP under the name СC-Rc accelerated the time of complete epithelialization of
wounds by 1.5 times. At the same time, the commercial NeuSkin-F comparison drug
(Eucare Pharmaceuticals (P) Limited, India) consisting of collagen without drug additives
showed very low wound healing properties in our studies due to poor adhesion. In this
regard, our CC-Rc, which has significantly better congruence and adhesion to the wound,
showed an excellent result, did not require additional fixation.