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  1. Home
  2. Volume 7, Issue 8
  3. Authors

Online ISSN: 2515-8260

Volume7, Issue8

Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis

    Dr.C. Devi Dr.R. Ravichandran Dr. Logeswari Selvaraj Dr.S. Ramesh Dr.T. Aarthipriya

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 4521-4527

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Abstract

Objectives: To test Rheumatoid factor (RF) IgM autoantibody in Patients with
Rheumatoid Arthritis by various methods like latex agglutination, Nephelometer and
ELISA. Comparative analysis of the sensitivity and specificity of the tests performed.
Materials and Methods: The study was conducted for a period of six months from June
2018 to November 2018 in a tertiary care hospital in Chennai. 90 patients attending
Rheumatology OPD or admitted in the ward with the diagnosis of Rheumatoid arthritis,
satisfying the inclusion and exclusion criteria were taken up for the study.
Inclusion criteria: Clinically diagnosed Rheumatoid Arthritis patient as per revised ACR
1987 classification criteria. Duration of symptoms (1yr- early (RA) 1 Yr. (Established RA).
Exclusion Criteria: Those with systemic connective tissue diseases like SLE, Scleroderma,
MCTD, Sjogren syndrome, those with chronic liver diseases, tuberculosis, subacute
Bacterial endocarditis, Pregnancy, Lympho reticular malignancies are excluded for the
study. Those with onset 16 years of age are also excluded. Under aseptic precautions about
3ml of blood was collected from each Patient. Rheumatoid factor (RF) IgM was tested for
each patient by all three methods Latex agglutination, Nephelometry and ELISA.
Results: IgM rheumatoid factor (RF) was detected in the sera of 90 patients with
Rheumatoid Arthritis. The percentage of positivity for Rheumatoid factor by Latex
agglutination, ELISA and Nephelometry were 41,64&60 respectively. Sensitivity and
Specificity of ELISA when compared to nephelometry were 63 & 33% followed by latex
agglutination 41 &59%.
Conclusion: Though nephelometry is considered as gold standard, in this study ELISA was
highly sensitive more even than nephelometry in Rheumatoid factor detection followed by
latex agglutination.
Keywords:
    Rheumatoid factor Latex agglutination Nephelometry ELISA
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(2021). Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis. European Journal of Molecular & Clinical Medicine, 7(8), 4521-4527.
Dr.C. Devi; Dr.R. Ravichandran; Dr. Logeswari Selvaraj; Dr.S. Ramesh; Dr.T. Aarthipriya. "Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis". European Journal of Molecular & Clinical Medicine, 7, 8, 2021, 4521-4527.
(2021). 'Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis', European Journal of Molecular & Clinical Medicine, 7(8), pp. 4521-4527.
Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis. European Journal of Molecular & Clinical Medicine, 2021; 7(8): 4521-4527.
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