European Journal of Molecular & Clinical Medicine

Abstract

This study was designed to evaluate the effect of Lycopene powder and /or vitamins AD₃Eadding to growing Awassi Male Lambs on bloodphysiologicalTraits.
Twenty healthy Awassi Male Lambs were selected for this study at approximately (4-5) month of age and average body weight (BW) of 19 kg and were reared in the livestock farm, experimental feeding was continued from 3/12/2019 to 2/3/2020 in addition to 15 day as adaptation period.
After adaptation period, lambs were randomly divided into four groups (5 lambs/group), The first group used as a control with no treatment, the second group received orally a solution of mixed vitamins (AD₃E) (A 20.000 IU, vitamin D₃ 10.000 IU and vitamin E 60 mg /lamb) biweekly, the third group, usedLycopene powder, (1% of theconcentrate diet) and same dose of (AD₃E) and the fourth group, usedLycopene powder (1%  of theconcentrate diet). However, all groups were fed a concentrate diet (2.5%DM of live body weight) in addition to alfalfahay (1 kg / head/day
. Bloodsamples were collected from jugular vein monthly for blood parameters measurement include: hemoglobin (Hb), packed cell volume (PCV),totalserumprotein(TSP),Albumineand Globulinewere determined.
The results revealed that:-
1-There was no significant difference between groups of lambs in PCV% throughout the experimental period. There was significant increase (P≤0.05) in Hb concentration of G4   at wk6 compared with G2, while, there were significant differences (P ≤ 0.05)betweenwk6 and wk0in G3.
2-There was a significant (P ≤ 0.05) increased   in WBC count of the treated groups G4, G3, G2  respectively  at wk12 compared with G1 and  there was a significant(P≤ 0.05) increased  in  WBC count of  G2 at wk12  compared with wk0 and wk6 respectively.Also,G3was significantly(P≤ 0.05) increased at wk12 and wk6 compared with wk0.
3-Nutrophile cells % was significantly(P≤0.05) increased in G4 at wk6 compared with G3 and also, G2 at wk12 compared with G3, while G3 was significantly(P ≤0.05)increased   at week0 compared with Wk6. Lymphocyte cells% was significantly(P≤0.05)  increased   in G3 at wk12 compared with G2, while there were non-significant differences between groups during wk0 and wk6, and  between the three periods in all groups.There was non-significant difference in the Monocytes between all groups of lambs during the whole period of experiment.The Esinophiles of G3 was significantly  (P≤0.05) increased at wk12 compared with G2  and also  at wk6 and  wk12  compared with  G4 at wk6 and wk12. In addition, Esinophiles in G2 was significantly(P≤0.05)  increased at wk6 compared with wk0 and wk12,also in G3 was significantly(P≤0.05) increased at wk6 compared with wk0. Basiophile cells% showed that there was significant(P≤0.05) increased in G2 at wk12 compared with G3 and G4, while inG4 was significantly (P ≤0.05) increased at wk6 compared with wk12.
4-The results ofTSPshowed that there was significant increase (P≤0.05)betweenG4 and G2 at wk12,whileG4increased significantly (P ≤ 0.05)at wk12,compared with other periods wk0 and  wk6 respectively, also G3 compared  with wk0. Albumine level in G1was significantly increased at wk6 compared with G2,G3 and G4, while at the same week, G2 and G3 were significantly increasedcompared with other two periods. There was significant increase (P ≤0.05)in serum Globuline in G1 compared with G2  at wk0. But,G1 showed a significant decrease (P≤0.05)  at wk6 in comparison with wk0 and wk 12. Also, G3 decreased significantly (P≤0.05) at wk6 in contrast with wk12, while G4 increased significantly (P≤0.05) at wk12 compared with wk0.
4-The results of the Lipids profile showed that Cholesterol  was  significantly (P≤ 0.05) increased in G3 at wk0 compared with G1,G2 and G4 respectively.Also,in G3 was significantly (P≤0.05) increased at wk0 compared with wk6,while in G4 was significantly (P≤0.05) increased at wk12 compared with wk0.However, Triacylglycerol, LDL  concentration of the animals in treated groups was significantly (P≤0.05) decreased compare with control group. While, The serum HDL for treated groups was significantly higher than G1.
In conclusion,adding of the feed with Lycopene powder and vitamins AD₃E to growing Awassi Male Lambs improvedblood traits.
This study was designed to evaluate the effect of Lycopene powder and /or vitamins AD₃Eadding to growing Awassi Male Lambs on bloodphysiologicalTraits.
Twenty healthy Awassi Male Lambs were selected for this study at approximately (4-5) month of age and average body weight (BW) of 19 kg and were reared in the livestock farm, experimental feeding was continued from 3/12/2019 to 2/3/2020 in addition to 15 day as adaptation period.
After adaptation period, lambs were randomly divided into four groups (5 lambs/group), The first group used as a control with no treatment, the second group received orally a solution of mixed vitamins (AD₃E) (A 20.000 IU, vitamin D₃ 10.000 IU and vitamin E 60 mg /lamb) biweekly, the third group, usedLycopene powder, (1% of theconcentrate diet) and same dose of (AD₃E) and the fourth group, usedLycopene powder (1%  of theconcentrate diet). However, all groups were fed a concentrate diet (2.5%DM of live body weight) in addition to alfalfahay (1 kg / head/day
. Bloodsamples were collected from jugular vein monthly for blood parameters measurement include: hemoglobin (Hb), packed cell volume (PCV),totalserumprotein(TSP),Albumineand Globulinewere determined.
The results revealed that:-
1-There was no significant difference between groups of lambs in PCV% throughout the experimental period. There was significant increase (P≤0.05) in Hb concentration of G4   at wk6 compared with G2, while, there were significant differences (P ≤ 0.05)betweenwk6 and wk0in G3.
2-There was a significant (P ≤ 0.05) increased   in WBC count of the treated groups G4, G3, G2  respectively  at wk12 compared with G1 and  there was a significant(P≤ 0.05) increased  in  WBC count of  G2 at wk12  compared with wk0 and wk6 respectively.Also,G3was significantly(P≤ 0.05) increased at wk12 and wk6 compared with wk0.
3-Nutrophile cells % was significantly(P≤0.05) increased in G4 at wk6 compared with G3 and also, G2 at wk12 compared with G3, while G3 was significantly(P ≤0.05)increased   at week0 compared with Wk6. Lymphocyte cells% was significantly(P≤0.05)  increased   in G3 at wk12 compared with G2, while there were non-significant differences between groups during wk0 and wk6, and  between the three periods in all groups.There was non-significant difference in the Monocytes between all groups of lambs during the whole period of experiment.The Esinophiles of G3 was significantly  (P≤0.05) increased at wk12 compared with G2  and also  at wk6 and  wk12  compared with  G4 at wk6 and wk12. In addition, Esinophiles in G2 was significantly(P≤0.05)  increased at wk6 compared with wk0 and wk12,also in G3 was significantly(P≤0.05) increased at wk6 compared with wk0. Basiophile cells% showed that there was significant(P≤0.05) increased in G2 at wk12 compared with G3 and G4, while inG4 was significantly (P ≤0.05) increased at wk6 compared with wk12.
4-The results ofTSPshowed that there was significant increase (P≤0.05)betweenG4 and G2 at wk12,whileG4increased significantly (P ≤ 0.05)at wk12,compared with other periods wk0 and  wk6 respectively, also G3 compared  with wk0. Albumine level in G1was significantly increased at wk6 compared with G2,G3 and G4, while at the same week, G2 and G3 were significantly increasedcompared with other two periods. There was significant increase (P ≤0.05)in serum Globuline in G1 compared with G2  at wk0. But,G1 showed a significant decrease (P≤0.05)  at wk6 in comparison with wk0 and wk 12. Also, G3 decreased significantly (P≤0.05) at wk6 in contrast with wk12, while G4 increased significantly (P≤0.05) at wk12 compared with wk0.
4-The results of the Lipids profile showed that Cholesterol  was  significantly (P≤ 0.05) increased in G3 at wk0 compared with G1,G2 and G4 respectively.Also,in G3 was significantly (P≤0.05) increased at wk0 compared with wk6,while in G4 was significantly (P≤0.05) increased at wk12 compared with wk0.However, Triacylglycerol, LDL  concentration of the animals in treated groups was significantly (P≤0.05) decreased compare with control group. While, The serum HDL for treated groups was significantly higher than G1.
In conclusion,adding of the feed with Lycopene powder and vitamins AD₃E to growing Awassi Male Lambs improvedblood traits.