ORGANOGENIC RESPONSE THROUGH CALLUS PHASE IN FEW MULBERRY (Morus spp.) VARIETIES
European Journal of Molecular & Clinical Medicine,
2021, Volume 8, Issue 2, Pages 1696-1707
Abstract
The experiment was conducted to know the of micropropagation efficiency of Tr10, Vishala, M5 and G2 mulberry (Morus spp.) varieties using leaf and nodal explants. Regeneration and multiple shoot formation were achieved from the callus phase from four mulberry varieties via Tr10, Vishala, M5 and G2. MS medium fortified with different concentrations and combinations of growth hormones were used, such as alone 2, 4 D (0.5 mg/l - 2.5 mg/l) and in combination with BAP (0.5 mg/l and 1.0 mg/l) for callus initiation from leaf and nodal explants. Shoot buds developed from callus were transferred to the multiplication medium containing BAP (0.5 mg/l – 3.0 mg/l) and NAA (0.1 mg/l) combination of growth hormones. The micro shootlets were subcultured to obtain sufficient growth of the shoots. Then shootlets were transferred to the rooting media containing NAA and IBA (0.5 mg/l – 3.0 mg/l). The results indicate that explants inoculated in alone 2, 4 D has enhanced the callus formation percentage. The best callusing was observed from nodal explants in alone 2, 4 D at 2.0 mg/l concentration in most of the varieties. Whereas the leaf explants did not show any further regeneration and proliferation was ceased in callus phase
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