In Vitro Regeneration Efficiency Of Few Diploid And Triploid Mulberry (Morus Spp.) Varieties
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 11, Pages 6344-6360
AbstractThe experiment was carried out to study the of micropropagation efficiency in few diploid and triploid mulberry (Morus spp.) varieties i.e S13, RFS135, Tr10, Vishala, M5 and G2, using axillary buds as explants. MS medium fortified with different concentrations and combinations of growth hormones were used. Alone BAP and kinetin were used in initiation media. BAP 2.0 mg/l was found to be most effective in inducing sprouting in most of the varieties. Shoots initiated from the nodal explants were transferred to the multiplication medium containing BAP (0.5 mg/l – 2.5 mg/l) + NAA (0.5 mg/l and 1.0 mg/l) and BAP (0.5 mg/l – 2.5 mg/l) + kinetin (0.5 mg/l and 1.0 mg/l) combinations of growth hormones. BAP 2.0 mg/l in combination with kinetin 1.0 mg/l was efficient in multiple shoots formation. The microshootlets were subcultured to obtain sufficient growth of the multipleshoots. The results indicated that explants inoculated in alone BAP has showed high percent of shoot initiation. For multiple shoot formation BAP in combination with kinetin was found to be effective in inducing efficient multiple shoots from the initiated shoots. Between two auxins tested for root initiation, NAA found to induced highest percentage of rooting compared to IBA.
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