Introduction: Hematoxylin and Eosin staining is the globally practiced staining technique for histology and histopathology studies. Hematoxylin is a natural stain while Eosin is a synthetic dye. Despite all its uses and advantages in the field, Eosin Y is considered hazardous to health. Accordingly, the goal of this study is to find an alternative to Eosin, and develop the extract of Curcuma Longa L. (Turmeric) as annatural and eco friendly alternative staining solution to eosin in the histopathological staining procedure. Aim and Objectives: To analyse staining ability of turmeric extract as a potent histological stain and to compare staining ability of turmeric extract with that of eosin. Materials and Method: 30 Formalin fixed paraffin embedded blocks were retrieved and 2 slides were prepared from each block. One slide was stained with conventional Hematoxylin and Eosin staining method and the other with Hematoxylin and turmeric stain. Stained slides were evaluated with scoring criteria by independent obsever and statistical analysis was done. Results: C.longastains the tissues in different shades of yellow color and there were no statistical significant differenceseen in between Haematoxylin& Eosin stain and Haematoxylin& Turmeric stain. Less time was required for haematoxylin and turmeric staining procedure (40- 45 minutes) compared to conventional staining (60-65 minutes). Conclusion: Turmeric extract has the advantage of being non-toxic, non-inflammable, non-hazardous, economical, and easy to handle. So the turmeric stain can be used as an alternative to eosin in routine histopathological procedures.