BACKGROUND OF THE STUDY: Urinary tract infection is the major public health problem in terms of morbidity and financial cost and in cures the highest total health care cost among urological disease. The urinary tract infection is an infection in any part of the urinary system. One of the most common bacterial infections amongst in adolescents and prevalence of infection depends largely on age and gender. Female are most at the risk of urinary tract infection. OBJECTIVE: To assess knowledge and self-reported practices regarding prevention of urinary tract infection. METHOD AND MATERIAL: A quantitative research approach with non- experimental descriptive study was adopted in this study. The Stratified random sampling technique was used to select the subject for the study. Data collected from 100 subjects were analyzed by descriptive and inferential statistics using SPSS version 22.