A new series of 3-(4-substituted phenyl)-2-phenyl-4-(3H)-quinazolinones 2(a-e) were synthesized by reacting 2-phenyl-4H-3-1-benzoxazin-4-one (1) with various primary amino compounds. The 2-phenyl-4H-3-1-benzoxazin-4-one (1) was prepared from anthranillic acid and benzoyl chloride. The structure of the title compounds was confirmed based on IR, 1H NMR, and Mass data. The compounds were screened for their antimicrobial activity and few of them were found to possess promising antimicrobial activity.