Aim: The aim of the present study to evaluate the minerals, urea, and creatinine in chronic renal failure. Methods: A case-control study was conducted in the Department of Biochemistry, Vardhman Institute of Medical Sciences (VIMS), Pawapuri , Nalanda, Bihar, India and dr.Siingh Diagnostic ,Nawada,Bihar, for 1 year, A total of 80 patients in the age group of 25- 65 years were included in this study. 40 patients were taken as a control group (Group A) and 40 clinically diagnosed cases of chronic renal failure patients based on the creatinine value > 7.0 mg/dl as cases (Group B). The parameters RBS, creatinine, urea, sodium, potassium, magnesium phosphorous, iron and calcium was analyzed by Automated Analyzer. Results: The age of the patients was 25-65 years. Comparison between the controls and cases (CRF) of RBS, creatinine, urea, sodium, potassium, magnesium phosphorous, iron and calcium parameters have shown a statistically significant difference in all the parameters, pvalue <0.05. A comparison of CRF subjects based on gender in persons undergoing dialysis has shown no statistically significant difference in all the parameters, p-value > 0.05 Comparison of groups RBS, creatinine, urea, sodium, potassium, magnesium, phosphorus, iron and calcium parameters in CRF with hypertension versus CRF with both hypertension and DM cases have shown statistically no significant difference in all the parameters, p-value > 0.05. We observed increase in the levels of serum calcium mean value 8.14±0.53 (mg/dl) and decrease in serum potassium mean value 4.71±0.77 (mEq/L), in male CRF subjects compared with female CRF subjects and has shown no statistical significance. we observed elevated serum creatinine 10.9±4.42 (mg/dl) in CRF patients with hypertension compared with CRF subjects with both hypertension and DM serum creatinine 9.88 ± 4.43 (mg/dl) and have shown no statistical significance. Conclusion: The elevated serum phosphate, magnesium and decreased serum calcium, iron are concerning with CRF. Serum phosphorous, magnesium, serum calcium and iron play important role in renal profile.