Aim: The study was aimed at exploring oxidative stress induced during kidney damage by streptomycin and benefit of antioxidant vitamin C. Material and methods: This was a Retrospective observational and analytical study was done in the Department of Medicine, Anugrah Narayan Magadh Medical College and Hospital, Gaya, Bihar for 18 months. Total 40, around 18 weeks old adult, male albino wistar strain rats were used with an average weight of around 170 gms. There were 4 groups, with 10 rats in each. The drug administered was as follows: Group I Control group, was given saline 0.3 ml i.p. per day. Group II was given Vitamin C 0.2mg/kg/day i.p. Group III was given streptomycin 100 mg/kg/day . While group IV received streptomycin and vitamin C with 100mg/kg/day i.m. and 0.2 mg/kg/day i.p. respectively. Blood urea and s. creatinine were measured to estimate renal damage. To measure oxidative stress Glutathione peroxidase (GPx), Glutathione reductase (GR) and Total antioxidant status (TAS) were analyzed. Results: There was an increase of GPx by 10% in vitamin C group, while GR and TAS were increased by 5% and 29% respectively as compared to control. In streptomycin treated group all values were significantly decreased as can be seen from tables. Co administration of vitamin C significantly prevented oxidative stress and reduced formation of free radicals. There was significant rise in the level of blood urea and s.creatinine in streptomycin treated group. When vitamin C was co-administered changes were less evident. Conclusion: We concluded that the antioxidant vitamin C has shown predictive benefit in streptomycin induced nephrotoxicity.