Objective: This original research aimed to assess the knowledge and awareness levels of dental professionals and patients regarding the novel COVID-19 variant, JN1. Methods: A cross-sectional study was conducted involving 400 participants, comprising 200 dental professionals and 200 patients attending dental clinics. A structured questionnaire was utilized to evaluate participants' understanding of the JN1 variant, encompassing its transmission dynamics, preventive measures, and treatment options. Descriptive and inferential statistical analyses were employed to interpret the data. Results: Significant disparities in knowledge levels were observed between dental professionals and patients. While dental practitioners exhibited moderate to adequate understanding of the JN1 variant, patients displayed substantial gaps in awareness, particularly concerning preventive measures and transmission dynamics. Healthcare providers were identified as the primary source of information for patients. Conclusion: The study highlights notable knowledge gaps among patients regarding the JN1 variant, emphasizing the need for targeted educational interventions and improved communication strategies. Addressing these disparities is crucial for enhancing public awareness, promoting preventive measures, and mitigating the impact of the JN1 variant within dental healthcare settings and the broader community.