Nanotechnology is a science involved within the design, synthesis, characterization and applications of materials and their components at nanoscale dimensions. The present study has been done to assess the knowledge and awareness about nanotechnology in dentistry among undergraduate dental students by distributing 12 questionnaires about nanotechnology among undergraduate dental students. The collected data was supported through SPSS software. The questions were based on knowledge necessary for nanotechnology in dentistry. Out of 100 participants' responses, 59% were aware of nanotechnology and only 67% of participants used nanomaterials within the clinical practice and 72% weren't using it. This study revealed that undergraduate dental students have significant knowledge about nanotechnology.