Endodontic file fracture is an uncommon event. It is important to assess the variations in root and root canal morphology before initiating any endodontic treatment .Occurrence of instrument fracture is reported to range between 0.3% and 16.2% . The aim of the study is to assess the association between age and teeth referred for retrieval of a separated instrument. In this retrospective study a total of 82000 patient records were reviewed and data related to instrument retrieval were extracted and tabulated for data analysis. Statistical analysis was done using SPSS software and chi square test was used to determine the occurrence of instrument fracture in patients undergoing root canal treatment based on age and teeth. Occurrence of instrument fracture was found to be highest in the 31 to 41 years age group and there is no relation between age and teeth referred for retrieval of separated instrument.