Background: Enterococci have emerged as an important cause of nosocomial infections, and antibiotic resistance Enterococcus is a major obstacle for treatment. Aim:to determine the prevalence of various enterococcus species and their antibiotic resistance pattern among urinary isolates. Materials and Methods: This observational study was carried out in the Department of Microbiology Jawaharlal Nehru Medical College and Hospital, Bhagalpur, Bihar, India for 15 months. A total of 220 Enterococcus species isolated from urine samples were included in the study. Only one isolate per patient was included in the study. Results: The present study shows a high incidence of enterococcal UTI among females (135) compare to males (85) So Male to Female ratio was 0.63. Isolation of enterococci was maximum among population between age group of 30- 40 (90) followed by 40-50 (36) and 20- 30 (30). Most common species found in present study was Enterococcus faecalis 130 (59.09%) followed by E. faecium 60 (27.27%), E. durans 20 (9.09%) and E. avium 10(4.54%). In present study the Ampicillin, Piperacillin, Ciprofloxacin, Norfloxacin, Nitrofurantoin, Gentamicin, Vancomycin and Linezolid antibiotic discs was tested by Kirby-Bauer disc diffusion method. Linezolid was most effective among all showed sensitivity for (88.18%), followed by vancomycin sensitive to (65%) on isolates, Nitrofurantoin sensitive to (51.36%)on isolates, Ampicillin sensitive to (36.36%) isolates, Piperacillin sensitive to (32.27%) isolates, and other was least effective. Conclusion: E. faecalis and E. faecium found to be the most prevalent species which confer resistance to various groups of antibiotics. E. faecium found to be more resistant species then E. faecalis.