Objective: This study was aimed to assess bacterial isolates and their drug susceptibility patterns from patients with pus and/or wound discharge .In spite of advances in control of infections, the widespread uses of antibiotics, together with the length of time over which they have been available have led to major problems of resistant organisms contributing to morbidity and mortality. Methods: A retrospective study was conducted at SMS medical college Jaipur where samples from all individuals who present with pus and/or wound discharge was collected and processed, from March 2019 to 7502February 2021. Result: A total of 2237 study subjects were included in the study with bacterial isolation rate of 1902 (85.02%). Of all, 1116 (58.6%) were males.448 (23.56%) of the isolates were gram positive and 1454 (76.44%) were gram negative. Among the isolates ,Pseudomonas was 33.59%, Klebsiella spp. 19.72% ,Esherichia coli 11.82% , Staphylococcus aureus 10.15%, Coagulase Negative staphylococci 10.05% and enterococcus spp. 3.36%. Conclusion: High prevalence of bacterial isolates were found; PSEUDOMONAS being the dominant. Most of the isolates were resistant to many of the antibiotics tested Antibiotic susceptibility test is necessary for effective control of wound infections.