Infection with H. pylori bacteria is considered one of the most common health problems in recent years, affecting half of the world's population. Many studies have been conducted on the effects of this infection. Therefore, our study aimed to study the change in the secretion of the adrenal cortex and its relationship to blood sugar levels, in addition to studying some physiological parameters in affected women visiting outpatient clinics in Al- Diwaniyah. 34 blood samples were collected, then the level of cortisol in the blood was measured, in addition to the blood sugar level and physiological blood parameters, as well as a bacterial study that included the isolation and diagnosis of these bacteria. The results showed a decrease in the level of cortisol in the patients' blood, while blood sugar did not show a significant increase. There was also a significant increase in the number of white blood cells and a decrease in hemoglobin and red cell indicators. The advancements made this year in the diagnosis of Helicobacter pylori mostly focused on endoscopy and molecular approaches. All the specimens were obtained and subjected to standard cultural and biochemical assays. Only 29 specimens successfully proliferated and were sustained on a specified culture medium. There are 16 females, accounting for 47.05% of the total, and 13 males, accounting for 38.23%. The presence of Hp1 and Hp2 specific sectors of 16s rRNA clearly observed using genetic detection techniques on a UV transilluminator at a length of 109 bp.