Background: Objectives: To determine the microbiological profile and antimicrobial susceptibility pattern of bacterial isolates from aural swabs in patients with CSOM. Materials and Methods: A total of 142 patients of CSOM with unilateral or bilateral ear discharge who attended the outpatient department from March 2020 to November 2020 were included. Results: Among 142 patients included in the study, most common age group was 11-20 years. CSOM was found to be more common in females (56.33%) than in males (43.66%). The most common bacterial isolate was Pseudomonas aeruginosa (45.8%) followed by Staphylococcus aureus (30.5%), Proteus mirabilis (13.8%), Acinetobacter baumannii (2.7%), Klebsiella pneumonia, Micrococcus, E. Coli, Citrobacter and Providentia species. Pseudomonas aeruginosa was found to have maximum sensitivity to Polymyxin B (87.8%) and least sensitive to amoxicillin (15.1%). Among Staphylococcus aureus (87% MRSA and 13% MSSA) maximum isolates were sensitive to piperacillin+tazobactam (90.9%) and least sensitive to amoxicillin (13.6%).