Background: Fine needle aspiration cytology (FNAC) is safe reliable, cost effective & minimally invasive method for evaluating salivary gland lesions owing to its easy accessibility & superficial location. The objective of this study was cytological study of salivary gland lesion. Methods: This prospective study was carried out in the Department of Pathology, Jhalawar Medical College and SRG Hospital, Jhalawar. The study was comprise of all patients of salivary gland lesions of either sex and age admitted/attending in our hospital after considering inclusion and exclusion criteria. Results: The sensitivity of the cytopathological diagnosis was 64.29%, specificity was 95.45%, positive predictive value was 81.82%, and negative predictive value was 89.36, and accuracy was 87.93% considering histopathology as gold standard. Conclusion: FNAC as a safe, simple, rapid, reliable, cost-effective, and well tolerated preoperative procedure with minimal morbidity carried out in outpatient department yielding accurate diagnosis of inflammatory, benign, and malignant salivary gland lesions with good sensitivity and specificity.