Abstract. With the expeditious growth in digital technology, biometrics makes the highest level of security compared to conventional methods like passwords and PIN numbers. Biometric is the measurement and statistical analysis of a user's unique physical (Fingerprint, Iris, Hand, DNA, Face) or behavioural (Voice, Sign, Keystroke) characteristics. Among all the Biometric techniques, Fingerprint is distinctive for every person and it can be utilized as a technique for individual identification. In this paper, a fingerprint template encryption scheme based on DNA Encoding and Genetic Algorithm is proposed to secure fingerprint templates. The fingerprint template is processed by feature extraction and the features are encrypted using DNA cryptographic algorithm .XOR operation is performed for the second layer of authentication. During the decryption process, we obtain the feature vector which matches with the input feature vector and the user input image is retrieved as final output.