Background:Elastomers are routinely used as final impression materials. This study was an attempt to determine cytotoxicity level of various elastomers using indirect method. Materials & Methods: In this studyPoly vinyl ether silicone (PVES) (EXA’lence light body), poly vinyl siloxane (PVS) (Flexceed light body and polyether (PE) impression material (Impregum) were classified Ito group I, II and III respectively. A total of 12 specimens were prepared. Dulbecco’s modified Eagle’s medium was used for growing mouse cell line NIH/3T3. Cytotoxicity level of allelastomers were measured with the test 3- (4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay. Results: The mean ± SD cell viability survival of cells at day 1 in group I was 25.4 ± 8.23, 106.2 ± 31.5 in group II and 121.4 ± 16.2 in group III. On day 3, it was 23.7 ± 7.21, 68.2 ± 32.7 and 99.4 ± 14.2 in group I, II and III respectively. On day 7, it was 24.8 ± 6.45, 19.4 ± 31.5 and 73.6 ± 15.8 in group I, II and III respectively. The difference was significant (P< 0.05). There wasdecline in the survival rate with Poly vinyl ether silicone as found on day 1,poly vinyl siloxane and polyether showed on 3rd and 7th day. Conclusion:Authors found that cell viability for poly vinyl siloxane(PVS) was highest as compared to other elastomeric impression material.